Fragile Nucleosome
Fragile Nucleosome is an international community of scientists interested in chromatin and gene regulation. Fragile Nucleosome is active in several spaces: one is the Discord server where several hundred scientists chat informally on scientific matters. You can join the Fragile Nucleosome Discord server. Another activity of the group is the organisation of weekly virtual seminars on Zoom. Our webinars are usually conducted on Wednesdays 9am Pacific time (5pm UK, 6pm Central Europe). Most previous seminars have been recorded and can be viewed at our YouTube channel. The schedule of upcoming webinars is shown below. Our third activity is the organisation of weekly journal clubs detailed at a separate page (Fragile Nucleosome Journal Club). Our fourth activity is the mentorship program for which you can sign up at the Discord server. If you want to be reminded about upcoming events (listed below) please subscribe to the mailing list here.
Organisers:
Christine Cucinotta (Fred Hutchinson Cancer Research Center)
Ben Martin (Harvard Medical School)
Matteo Perino (EMBL, Heidelberg)
Pravrutha Raman (Fred Hutchinson Cancer Research Center)
Ulduz Sobhiafshar (Boğaziçi University, Istanbul)
Vladimir Teif (University of Essex)
Xiaoli Sky Wu (Cold Spring Harbor Laboratory)
More details about the Fragile Nucleosome community can be found in our publication:
Cucinotta C.E., Martin B.J.E., Noé González M., Raman P., Teif V.B., Vlaming H. (2021) Strength is in engagement. EMBO Reports 22(5), e52612. | Open access article
Upcoming seminars
18
May 2022
9 am PST
12 pm EST
5 pm BST
6 pm CET
Nezha Benabdallah
DKFZ Heidelberg
Title talk: TBA
Karmella Haynes
Georgia Institute of Technology and Emory University
Rational Design of Reader-Effectors: Synthetic Biology Meets Chromatin Epigenetics
Past seminars:
1
April 2020
Hiten Madhani: Epigenetic memory over geological timescales
9:00am (Pacific time)
UCSF, USA
8
April 2020
Toshio Tsukiyama: Regulation of Quiescence through Chromatin
9:00am (Pacific time)
Fred Hutchinson Center, USA
15
April 2020
Susan Gasser: Histone turnover in response to DNA damage: part of the checkpoint response
9:00am (Pacific time)
Friedrich Miescher Institute, Switzerland
29
April 2020
Cynthia Wolberger: Mechanisms of cross-talk between histone ubiquitination and methylation
9:00am (Pacific time)
Johns Hopkins University, USA
6
May 2020
Karolin Luger: An Epi(genetic) Battle: Nucleosomes and the Nuclear Machinery
9:00am (Pacific time)
University of Colorado Boulder, USA
13
May 2020
Tracy Johnson: A histone tale: RNA splicing and coordinated control of gene expression
9:00am (Pacific time)
UCLA, USA
20
May 2020
Sheila Teves: Transcriptional memory and dynamics in embryonic stem cells
9:00am (Pacific time)
University of British Columbia, Canada
27
May 2020
Fred van Leeuwen: Unraveling chromatin mechanisms by DNA barcoding
9:00am (Pacific time)
Netherlands Cancer Institute
3
June 2020
Alistair Boettiger: Visualizing genome folding and gene expression in development
9:00am (Pacific time)
Stanford University, USA
11
June 2020
Don and Ada Olins: Nuclear architectural plasticity and stability in a myeloid cell
9:00am (Pacific time)
University of New England, USA
17
June 2020
Rob Beagrie (Douglas Higgs group): In vivo dynamics of transcription, chromatin and genome organization during mouse erythroid differentiation
9:00am (Pacific time)
University of Oxford, UK
17
June 2020
Siyuan (Steven) Wang: Multiplexed imaging of nucleome architectures in single cells of mammalian tissue
9:30am (Pacific time)
Yale University, USA
24
June 2020
Angelika Feldmann (Rob Klose Lab): Cohesin disrupts polycomb-dependent chromosome interactions in ESCs
9:00am (Pacific time)
University of Oxford, UK
24
June 2020
Andrew Pospisilik: Epigenetics, bistability and probabilistic metabolic disease
9:30am (Pacific time)
Van Andel Institute, USA
1
July 2020
Apoorva Baluapuri (Elmar Wolf Lab): MYC Recruits SPT5 to RNA Pol II to Promote Processive and Directional Transcription Elongation
9:00am (Pacific time)
Biozentrum Würzburg, Germany
1
July 2020
Gabe Zentner: Transcription start sites earn their STRIPEs
9:30am (Pacific time)
Indiana University, USA
8
July 2020
Natalia Kochanova (Bill Earnshaw Lab): A multi-layered structure of the interphase chromocenter revealed by proximity-based biotinylation
9:00am (Pacific time)
University of Edinburgh, UK
8
July 2020
Tobias Warnecke: Histones in archaea
9:30am (Pacific time)
Imperial College London, UK
15
July 2020
Aleksandra Skrajna (Robert McGinty Lab): Deciphering the nucleosome interactome
9:00am (Pacific time)
University of North Carolina, USA
15
July 2020
Emma Morrison: Nucleosome Composition Regulates the H3 Tail Conformational Ensemble and Accessibility
9:30am (Pacific time)
Medical College of Wisconsin, USA
22
July 2020
Daria Shlyueva (Kristian Helin Lab): Integrated approach to study cell cycle-dependent chromatin dynamics
9:00am (Pacific time)
MSKCC, USA
22
July 2020
Vijay Ramani: Massively multiplex single-molecule oligonucleosome profiling
9:30am (Pacific time)
UCSF, USA
29
July 2020
Lucas Farnung (Patrick Cramer Lab): Chromatin transcription: cryo-EM structures of Pol II-nucleosome and nucleosome-CHD complexes
3:00am (Pacific time)
MPI-BPC Göttingen, Germany
29
July 2020
Hitoshi Kurumizaka: Dynamic structural changes in the nucleosome during gene regulation
3:30am (Pacific time)
University of Tokyo, Japan
5
August 2020
Nathan Gamarra (Geeta Narlikar Lab): Allosteric dynamics in the nucleosome acidic patch control the chromatin remodeler SNF2h
9:00am (Pacific time)
UCSF, USA
5
August 2020
Felix Mueller-Planitz: The biogenesis and function of nucleosome arrays
9:30am (Pacific time)
TU Dresden, Germany
12
August 2020
Henry Fabian Thomas (Christa Buecker Lab): Genetic dissection of the Fgf5 enhancer cluster
9:00am (Pacific time)
MPL Vienna, Austria
12
August 2020
Srinivas Ramachandran: Cooperative binding of transcription factors is a hallmark of active enhancers
9:30am (Pacific time)
University of Colorado, USA
19
August 2020
Madeline Keenen: Static DNA compaction by the dynamic binding protein Heterochromatin Protein-1
9:00am (Pacific time)
UCSF, USA
19
August 2020
Sebastian Marquardt: Epigenetics of Genomic Punctuation
9:30am (Pacific time)
University of Copenhagen, Denmark
26
August 2020
Rodrigo Arzate: In situ dissection of domain boundaries in Drosophila
9:00am (Pacific time)
UNAM, México
26
August 2020
Yvonne Fondufe-Mittendorf: Gene expression reprogramming in response to an environmental cue
9:30am (Pacific time)
University of Kentucky, USA
2
September 2020
Kelsey Lynch: Adenovirus protein VII hijacks host chromatin factors to undermine the cell cycle
9:00am (Pacific time)
Fred Hutchinson Center, USA
2
September 2020
Siavash Kurdistani: Histones as facilitators of eukaryogenesis
9:30am (Pacific time)
UCLA, USA
9
September 2020
Giacomo Corleone: AATF-induced chromatin shifts actively sustain Multiple Myeloma progression
9:00am (Pacific time)
IRCCS National Cancer Institute, Italy
9
September 2020
Effie Kostareli: BTK signalling and enhancer landscape in Chronic Lymphocytic Leukaemia
9:30am (Pacific time)
Queen’s University Belfast, UK
23
September 2020
Danny Conrad: Sample Multiplexing in Single-Cell Epigenomic Assays
9:00am (Pacific time)
UCSF, USA
23
September 2020
Naama Barkai: Profiling nucleosome exchange using cell-intrinsic ‘timer’
9:30am (Pacific time)
Weizmann Institute, Israel
30
September 2020
Donya Shodja: Expansion of a pre-existing signaling center underlies the evolution of a morphological novelty in the Drosophila genitalia
9:00am (Pacific time)
University of Pittsburgh, USA
30
September 2020
Mary Gehring: Plant gene imprinting dynamics across multiple scales
9:30am (Pacific time)
MIT, USA
7
October 2020
Elizabeth Ing-Simmons: Independence of 3D chromatin conformation and gene regulation during Drosophila dorsoventral patterning
9:00am (Pacific time)
MPI Molecular Biomedicine, Muenster
7
October 2020
Josh Tycko: High-throughput discovery and characterization of human transcriptional effectors
9:30am (Pacific time)
Stanford, USA
14
October 2020
Maggie Chassé: Mithramycin inhibits SWI/SNF to induce promoter reprogramming and differentiation in rhabdoid tumor
9:00am (Pacific time)
Van Andel Institute, USA
14
October 2020
Sarah Hainer: Profiling of Pluripotency Factors in Single Cells and Early Embryos
9:30am (Pacific time)
University of Pitsburghs, USA
21
October 2020
Mathias Nielsen: The role of accessory proteins in epigenetic switching at a plant locus
9:00am (Pacific time)
John Innes Centre, UK
21
October 2020
Argyris Papantonis: The role of RNA polymerase II in genome reorganisation after mitosis and its link to loop extrusion
9:30am (Pacific time)
University of Göttingen, Germany
28
October 2020
Inwha Baek: Single molecule studies of RNA polymerase II transcription initiation and elongation
9:00am (Pacific time)
Harvard Medical Schoola, USA
28
October 2020
Karen Arndt: Analysis of proteins at the interface of chromatin and transcription
9:30am (Pacific time)
University of Pittsburgh, USA
4
November 2020
Yanfeng He: Elongin contributes to activator-dependent transcription
9:00am (Pacific time)
Stowers Institute, USA
4
November 2020
François Robert: Transcription and chromatin remodeler Chd1 join forces to convey FACT to genes
9:30am (Pacific time)
Montreal Clinical Research Institute
11
November 2020
Porfirio Quintero-Cadena: RNA Pol II length and disorder enable cooperative scaling of transcriptional bursting
9:00am (Pacific time)
Caltech, USA
11
November 2020
Montserrat Anguera: Dynamic X chromosome Inactivation Maintenance in the Immune System
9:30am (Pacific time)
University of Pennsylvania, USA
18
November 2020
Ália dos Santos: DNA damage alters nuclear mechanics through chromatin reorganisation
9:00am (Pacific time)
University of Sheffield, UK
18
November 2020
Genevieve Almouzni: Histone H3 variants and their challenges, in and out and back again
9:30am (Pacific time)
Institut Curie, France
25
November 2020
Joanna Achinger-Kawecka: 3D Epigenome Remodelling in the Endocrine-Resistant Breast Cancer
9:00am (Pacific time)
Garvan Institute, Australia
25
November 2020
Wendy Bickmore: Moving apart for activation and clustering for repression
9:30am (Pacific time)
University of Edinburgh, UK
13
January 2021
Maggie Chassé: Mithramycin induces promoter reprogramming and differentiation of rhabdoid tumor
9:00am (Pacific time)
Van Andel Research Institute, USA
13
January 2021
Fred Winston: Histone chaperones collaborate to control transcription, chromatin structure, and genome stability
9:30am (Pacific time)
Harvard Medical School, USA
27
January 2021
Christine Cucinotta: RSC primes the quiescent genome for hypertranscription during cell cycle re-entry
9:00am (Pacific time)
Fred Hutchinson Center, USA
27
January 2021
Folkert van Werven: Noncoding transcription, chromatin states and cell fate control
9:30am (Pacific time)
Francis Crick Institute, UK
10
February 2021
Manuel Rosa-Garrido: Temporal Chromatin Structural Remodeling During Heart Failure
9:00am (Pacific time)
UCLA, USA
10
Febbruary 2021
Lluis Morey: Defining the role of LSD1/CoREST in advanced breast cancer
9:30am (Pacific time)
University of Miami, USA
24
February 2021
Ariel Afek: The impact of DNA mismatches on protein–DNA interactions
9:00am (Pacific time)
Duke University, USA
24
Febbruary 2021
Pablo Navarro Gil: Should I stay or should I go, the mitotic clash
9:30am (Pacific time)
Institut Pasteur, France
10
March 2021
Madhura De: Tinkering with the linkers: a single-molecule FRET study of the linker-DNA and the linker histone on a nucleosome
9:00am (Pacific time)
DKFZ & HITS, Germany
10
March 2021
Eileen Furlong: Chromatin topology and its role in enhancer-promoter communication and embryonic development
9:30am (Pacific time)
EMBL, Germany
24
March 2021
Rafał Donczew: BET family members Bdf1/2 modulate global transcription initiation and elongation in Saccharomyces cerevisiae
9:00am (Pacific time)
Fred Hutchinson Center, USA
24
March 2021
Marjorie Brand: Transcriptional Control of Cell Fate Decisions
9:30am (Pacific time)
Ottawa Hospital Research Institute
7
April 2021
Joanna Jachowicz: Xist-mediated condensates amplify SHARP localization on the X chromosome to promote chromosome-wide transcriptional silencing
9:00am (Pacific time)
Caltech, USA
7
April 2021
Jennifer Mitchell: A flexible repertoire of transcription factor binding sites and a diversity threshold determines mammalian enhancer activity
9:30am (Pacific time)
University of Toronto, Canada
21
April 2021
James Hawley: Cis-Regulatory Element Hijacking Overshadows Topological Changes in Prostate Cancer
9:00am (Pacific time)
Princess Margaret Center, Canada
21
April 2021
Marco Trizzino: Inability to switch from ARID1A-BAF to ARID1B-BAF impairs exit from pluripotency and commitment towards neural crest differentiation in ARID1B-related neurodevelopmental disorders
9:30am (Pacific time)
Thomas Jefferson University, USA
5
May 2021
Linda Forero Quintero, “Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single copy gene”
9:00am (Pacific time)
Colorado State University, USA
5
May 2021
Mounia Lagha, “Transcriptional dynamics and memory during zygotic genome activation”
9:30am (Pacific time)
Institute of Molecular Genetics, Montpellier
12
May 2021
Julie Brind’Amour, “Maternally-inherited H3K27me3 inhibits enhancer commissioning and transcription in the early embryo”
9:00am (Pacific time)
University of British Columbia
12
May 2021
Petra Hajkova, “Epigenetic reprogramming and plasticity”
9:30am (Pacific time)
Imperial College London, UK
2
June 2021
Yadira Marie Soto-Feliciano, “Mechanisms of Transcriptional Regulation by Chromatin Adaptor Proteins”
9:00am (Pacific time)
Rockefeller University, USA
2
June 2021
Song Tan, “Recognition of the nucleosome by chromatin enzymes”
9:30am (Pacific time)
Pennsilvania State University, USA
16
June 2021
Colin Hammond, “Integrating Heat Shock Molecular Chaperones into the Histone Chaperone Network”
9:00am (Pacific time)
University of Copenhagen
16
June 2021
Motoki Takaku, “Chromatin invasion by pioneer factor and chromatin remodelers”
9:30am (Pacific time)
University of North Dakota
30
June 2021
Krishnendu Guin, “Spatial inter-centromeric interactions facilitated the emergence of evolutionary new centromeres”
9:00am (Pacific time)
Jawaharlal Nehru Centre
30
June 2021
Antoine Molaro, “Unusual Histones Mediate Parental Conflicts in Mammals”
9:30am (Pacific time)
Genetics, Reproduction and Development Institute
14
July 2021
Myron Evans: YBX1-mediated regulation of PRC2 controls embryonic brain development
9:00am (Pacific time)
St. Jude Children’s Research Hospital
14
July 2021
Leonie Ringrose: Take, remember, give back: what do epigenetic regulatory elements do, and are they all the same?
9:30am (Pacific time)
Humboldt University
28
July 2021
Yasuhiro Arimura: Cryo-EM structures of nucleosomes from interphase and metaphase chromosomes.
9:00am (Pacific time)
Rockefeller University, USA
28
July 2021
Lori Passmore: Mechanistic insight into mRNA cleavage and polyadenylation
9:30am (Pacific time)
MRC Laboratory of Molecular Biology
11
August 2021
Vignesh Kasinath: Structural Insights into the Recruitment and Activation of Gene Silencer PRC2
9:00am (Pacific time)
University of Colorado, USA
25
August 2021
Alicia Michael: Transcription factors interface with histones to specify DNA engagement
9:00am (Pacific time)
Friedrich Miescher Institute for Biomedical Research
25
August 2021
Archana Dhasarthy: There and back again: models to study the Epithelial-Mesenchymal transition
9:30am (Pacific time)
University of North Dakota, USA
8
September 2021
Hanneke Vlaming: INSERT-seq reveals sequence determinants of RNA polymerase II elongation
9:00am (Pacific time)
Harvard Medical School
8
September 2021
Yuan He: Structural Visualization of Chromatin Regulatory Complexes using Cryo-EM
9:30am (Pacific time)
Northwestern University, USA
6
October 2021
Ashwin Narain: Targeted protein degradation reveals a direct role of SPT6 in RNAPII elongation and termination
9:00am (Pacific time)
University of Wuerzburg, Germany
6
October 2021
Sevinc Ercan: Topoisomerases I and II promote condensin DC translocation on DNA at different scales
9:30am (Pacific time)
New York University, USA
20
October 2021
Tyler Weaver: Structural basis for APE1 processing DNA damage in the nucleosome
9:00am (Pacific time)
University of Kansas Medical Center
20
October 2021
Sylvia Erhardt: Centromeric chromatin regulation: a balancing act to maintain genome stability
9:30am (Pacific time)
Karlsruhe Institute of Technology
3
November 2021
Sandipan Brahma: Chicken & Egg of Nucleosome Dynamics: RNA Pol II modulates BAF (SWI/SNF) nucleosome remodeling
9:00am (Pacific time)
Fred Hutchinson Cancer Research Center
3
November 2021
Laura Banaszynski: Fine-tuning chromatin function with histone variant H3.3
9:30am (Pacific time)
UT Southwestern Medical Center
17
November 2021
Xiaoli S. Wu: Essential OCT11 cofactors in normal and malignant tuft cells
9:00am (Pacific time)
Cold Spring Harbor Laboratory
17
November 2021
Nadine Vastenhouw: The spark of life. Initiating transcription in embryos
9:30am (Pacific time)
University of Lausanne
1
December 2021
Leonie Smeenk: Selective requirement of MYB for oncogenic hyperactivation of a translocated enhancer in leukemia
9:00am (Pacific time)
Erasmus Medical Center
1
December 2021
Steven West: A good beginning makes a great ending
9:30am (Pacific time)
University of Exeter
12
January 2022
Samuel Corless: Locus specific protein profiling identifies novel properties of centromeres
9:00am (Pacific time)
KITP & Heidelberg University
12
January 2022
Stephen Buratowski: Visualizing transcription activation dynamics in vitro using single-molecule microscopy
9:30am (Pacific time)
Harvard Medical School
26
January 2022
Joaquina Delas: Two distinct modes of cis-regulation control cell specification in response to Shh during spinal cord development
9:00am (Pacific time)
Francis Crick Institute, UK
26
January 2022
Tatjana Sauka-Spengler: Reconstructing gene regulatory circuits in the vertebrate neural crest
9:30am (Pacific time)
Oxford University, UK
9
February 2022
Ineke Brouwer: Dynamic epistasis reveals how chromatin remodeling regulates transcriptional bursting
9:00am (Pacific time)
Netherlands Cancer Institute
9
February 2022
Geeta Narlikar: A hexasome is the preferred substrate of the INO80 chromatin remodeling machine
9:30am (Pacific time)
UCSF, USA
23
February 2022
Tim Klingberg and Agnieszka Pancholi: RNA polymerase II clusters form in line with surface condensation on regulatory chromatin
9:00am (Pacific time)
FAU; Karlsruhe Institute of Technology
23
February 2022
Fei (Xavier) Chen: Transcriptional regulation by Integrator-PP2A (INTAC)
9:30am (Pacific time)
Shanghai Cancer Center
9
March 2022
Waad Albawardi: Non-repetitive nucleosome arrays form disrupted and fragile chromatin fibers
9:00am (Pacific time)
University of Edinburgh
9
March 2022
Shabih Shakeel: Mechanistic Insights into the Fanconi Anemia DNA Repair Pathway
9:30am (Pacific time)
WEHI
23
March 2022
Jeff Hansen: A Test of the Pioneer Factor Hypothesis
9:00am (Pacific time)
Washington University in St. Louis
23
March 2022
Steve DeLuca: New insights into Polycomb silencing initiation revealed by an ancient alternative somatic lineage
9:30am (Pacific time)
Brandeis University
6
April 2022
Ben Weekley: The Role of Matrix Metalloproteinase 2 (MMP-2) Mediated H3NT Proteolysis in Transcriptional Regulation
9:00am (Pacific time)
University of Southern California, USA
6
April 2022
Hamed S. Najafabadi: Charting the landscape of in vivo methyl-cytosine recognition by transcription factors
9:30am (Pacific time)
McGill University, Canada
20
April 2022
Kanishk Jain: Acetylation-mediated histone tail accessibility governs the read-write mechanism of H3K4 methylation
9:00am (Pacific time)
UNC Chapel Hill, USA
20
April 2022
Emma Farley: Affinity optimizing enhancer variants disrupt development
9:30am (Pacific time)
UCSD, USA
4
May 2022
Allison Porman Swain: From chromatin to RNA and back again: Identifying unique mechanisms of regulation in Candida and lnkRNA HOTAIR
9:00am (Pacific time)
University of Colorado, USA
4
May 2022
Alan Underhill: Testing the limits of heterochromatin protein dynamics
9:30am (Pacific time)
University of Alberta, Canada